Fig. 11. Cd activates Akt in a ROS mediated pathway. (A) and (B). HT-29 cells were treated with Cd (100 nM) for 0, 10, 30 and 60 minutes and Akt phosphorylation levels were assessed by western blotting. (C) and (D): cells were pre-treated with NAC (10 mM) for 30 minutes followed by Cd (100 nM) for 60 minutes and Akt phosphorylation levels were determined by western blotting. Values are represented as mean ± SEM of n=3. * denotes p<0.05, ** denotes p<0.01.